| Atura Power |
| Biodiversity and Climate Action Collective Niagara |
| Brock University (Coordinating Committee Member) |
| City of Niagara Falls |
| City of Port Colborne |
| City of St. Catharines |
| City of Thorold |
| City of Welland |
| Community Potential (formerly Niagara Connects) |
| District School Board of Niagara |
| Enbridge Gas |
| Friends of Walker’s Creek |
| Grape Growers of Ontario |
| Greater Niagara Chamber of Commerce |
| Grimsby Environmental Network |
| Grimsby & District Chamber of Commerce |
| Heartland Forest Nature Experience |
| Mississaugas of the Credit First Nation |
| Niagara Birding Conservation and Tourism Collaboration |
| Niagara Catholic District School Board |
| Niagara Chapter Native Women |
| Niagara Chapter Trout Unlimited |
| Niagara College (Coordinating Committee Member) |
| Niagara Falls Nature Club |
| Niagara Home Builders’ Association |
| Niagara Parks Commission |
Niagara Peninsula Conservation Authority
(Coordinating Committee Member) |
| Niagara Peninsula Energy Inc. |
| Niagara Region (Coordinating Committee Member) |
| Niagara Region Metis Council |
| Ontario Plant Restoration Alliance/Canadian Wildlife |
| Ontario Power Generation |
| Pelham Advocates for Trees and Habitats |
| Rankin Renewable Power Inc. |
| Six Nations of the Grand River |
| South Niagara Chamber of Commerce |
| Speak for the Creek |
| St. Catharines Downtown Association |
| St. Catharines Environmental Alliance |
| St. Lawrence Seaway Management Corporation |
| Sustainability Leadership |
| Town of Fort Erie |
| Town of Grimsby |
| Town of Lincoln |
| Town of Niagara-on-the-Lake |
| Town of Pelham |
| Township of Wainfleet |
| Township of West Lincoln |
| Vineland Research |
| Walker Industries |
| 50by30 Niagara |